DL-Dithiothreitol

Catalog	BBC3483123
CAS	3483-12-3
Structure
Synonyms	1,4-Dithiothreitol
IUPAC Name	(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
Molecular Weight	154.25
Molecular Formula	C4H10O2S2
Canonical SMILES	C([C@H]([C@@H](CS)O)O)S
InChI	InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI Key	VHJLVAABSRFDPM-QWWZWVQMSA-N
Boiling Point	125 °C
Melting Point	41-44 °C (lit.)
Purity	98%
Density	1.04 g/ml
Solubility	In water, 4.94X10+5 mg/L at 25 °C (est);Freely soluble in water;Freely soluble in ethanol, acetone, ethyl acetate, chloroform, ether
Appearance	White powder
Application	DL-Dithiothreitol (DTT) is a versatile small-molecule redox reagent with a critical role in biotechnology, biology, and biochemistry. It functions primarily as a strong reducing agent, effectively breaking disulfide bonds and preserving sulfhydryl groups of proteins and other biomolecules. This attribute is especially valuable for preparing proteins for structural and functional analysis, such as reducing proteins before SDS-PAGE analysis. Furthermore, DTT is employed in clinical microbiology due to its efficacy in liquefying respiratory specimens and detaching biofilms from orthopedic prostheses, aiding in the prompt diagnosis of implant-related infections. Its solubility in water and various organic solvents, combined with its ability to maintain biomolecule stability, makes DTT an essential tool for supporting the viability of pathogens during the identification process and preventing fluorescence fading in labeled conjugates.
EC Number	222-468-7;240-263-0;248-531-9
pH	4.0-6.0 ( 0.1m, H₂O)
pKa	8.9
Vapor Pressure	1.28X10-4 mm Hg at 25 °C (est)